Effect of exendin-4 on the expression of NF-κB p65 and ICAM-1 in aorta endothelium of type 2 diabetic rats
Abstract：Objective To explore the influence of exendin-4 on antioxidation index in serum and the expression of nuclear factor Kappa B (NF-κB)p65 and intercellular cell adhesion molecule-1(ICAM-1) in aorta endothelium of type 2 diabetic rats and the possible mechanism. Methods The normal rats were injected with streptozocin(STZ) into abdominal cavity after high fat diet to es-tablish the model of type 2 diabetic rats. Forty SD male rats were randomly divided into normal control group,diabetic model group and groups treated with low, medium, and high dose of ex-endin-4. The treated groups were treated with intraperitoneal injection of exendin-4 for six weeks.Heart blood was taken for examining biochemical indexes including malondialdehyde(MDA), SOD and total antioxidation capability(T-AOC). The thoracic aorta was isolated carefully to observe the expression of NF-κB p65 and ICAM-1 in endothelium with immunohistochemical method. Re-sults Exendin-4 could decrease the level of MDA,but increase the levels of SOD and T-AOC in type 2 diabetic rats. Treatment with exendin-4 could decrease the expression of NF-κB p65. The medium and high dose of exendin-4 could decrease the expression of ICAM-1 (P<0.05). Conclu-sion Exendin-4 has function to protect aorta endothelium,the mechanism is related to the antiox-idation and restraining activation of NF-κB p65 and ICAM-1 in the blood vessel endothelium.
Keywords：diabetes mellitustype 2NF-kappa Bcell adhesion moleculesendothelial cellssuper-oxide dismutase
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 610-613 )