Effects of PEP-1-superoxide dismutase fusion protein on cardiomyocyte apoptosis in rat myocardium after ischemia-reperfusion
Abstract：Objective To explore the influence of PEP-1-SOD1 fusion protein on cardiomyocyte ap-optosis, expression of Bax and Bcl-2 proteins following myocardial ischemia-reperfusion in rats.Methods Forty rats were randomly divided into sham-operated group, ischemia-reperfusion group,PEP-1-SOD1 100,300 and 500 μg groups(8 rats in each group). Ischemia-reperfusion mod-el was established by left anterior descending coronary artery ligation for 1 h,followed by 2 h of reperfusion. Cardiomyocyte apoptotic index(AI),Bax and Bcl-2 in cardiomyocytes were detected with TUNEL and immunohistochemistry methods, respectively. Results The levels of AI,Bax and Bcl-2 proteins were low in sham-operated group. In. PEP-1-SOD1 groups, the levels of AI,Bax and Bax/Bcl-2 ratio were decreased significantly as compared with ischemia-reperfusion group.However,the level of Bcl-2 protein was up-regulated in PEP-1-SOD1 groups (P < 0.05,P< 0.01). Conclusion PEP-1-SOD1 can inhibit the cardiomyocyte apoptosis probably through the mechanisms of alleviating oxidative stress,down-regulating Bax and up-regulating Bcl-2 proteins.
Keywords：myocardial ischemiamyocardial reperfusionapoptosissuperoxide dismutase
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 603-605 )