Effect of Raphani semen extract on inflammatory response of diabetic periodontitis rats based on PI3K/AKT pathway
HONG You-zhi
WANG Yan
LIU Ya-li
MU Xin-ge
XUE Jun-zhe
LI Yan-hui
Abstract：Objective To explore the protective effect of Raphani semen extract on periodontal inflammation in diabetic periodontitis rat model and analyze the correlation between Raphani semen extract and PI3K/AKT signaling pathway. Methods Forty male Sprague-Dawley (SD) rats were selected using random sampling methods and then the subjects were initially divided into five groups and then categorized as follows:the blank control group (Group N),periodontitis control group (Group P),periodontitis Raphani semen treatment group (Group P+Q),diabetic periodontitis group (Group DP),and diabetic periodontitis Raphani semen treatment group (Group DP+Q),with eight animals in each group.Except group N,the periodontitis model of the other 4 groups was established by orthodontic wire periodontitis ligation combined with bacterial solution coating. Specifically,the DP group and DP+Q group received an additional intraperitoneal one-time injection of streptozotocin to establish the diabetic periodontitis rat model. After successful modeling,Raphani semen extract 2.5g/(kg·d) was administered by gavage in the P+Q and DP+Q groups,respectively,and an equal amount of saline was given to the N,P,and DP groups. The drug was administered continuously for 8 weeks,during which the general state of the rats was observed and the changes of fasting blood glucose in the rats were tracked.Gingival index (GI) and sulcus bleeding index (SBI) of rats were recorded before execution. After execution,the height of alveolar bone resorption was recorded;serum IL-6 and TNF-α levels were assessed using ELISA,while pathological and morphological alterations in rat periodontal tissues were examined via HE staining. and protein expression levels of PI3K and AKT in rat periodontal tissues were detected by Western blotting. Results The inflammation levels in the P and DP groups were significantly higher than those in the N group (P<0.05),with those in the DP group significantly higher than those in the P group (P<0.05). The inflammation level of the P+Q and DP+Q groups treated with Raphani semen extract was significantly lower than that of the P and DP groups,respectively (P<0.05). The expression of PI3K and AKT in the DP group was significantly lower than that of the N group (P<0.05). In the DP+Q group,the expression levels of PI3K and AKT were significantly higher than those in the DP group. (P<0.05). Conclusion Raphanus semen extract can improve inflammatory response and protect periodontal tissue in diabetic periodontitis rats based on PI3K/AKT signaling pathway.
Keywords：Raphani semen extractDiabetes mellitusPeriodontitisPhosphatidylinositol 3-kinaseProtein kinase B
Publication Date：2024-09-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 257-261,285 )