Preparation and evaluation of small intestinal submucosal composite nano-hydroxyapatite sponge
TAN Yi-wen
LIU Bing
LI Qian
HUANG De-zhi
ZHAO Yan-tao
HU Xian-tong
PANG Jian-liang
Abstract：Objective The study aims to develop a small intestinal submucosal (SIS) composite nano-hydroxyapatite (nHA) sponge material and evaluate its physical and chemical characteristics.This material is intended to be a scaffold for bone tissue engineering.Methods Four scaffolds were prepared using the freeze-drying method with varying ratios of SIS and nHA (1︰0,1︰1,1︰2,and 1︰4),which were divided into SIS group,SIS︰nHA=1︰1 group,and SIS︰nHA=1︰2 group and SIS︰nHA=1︰4 group.The physicochemical characteristics and biocompatibilities of these scaffolds were subsequently evaluated.Results Four scaffolds that composed of small intestinal submucosa (SIS) and nano-hydroxyapatite (nHA) were prepared resulting in white loose porous spongy materials.Porosity values were measured at approximately (93.01±3.14)％,(86.35±12.19)％,(81.28±3.07)％ and (68.12±4.22)％.With an increase in hydroxyapatite content within the scaffolds,porosity gradually decreased significantly (P<0.05).Water absorption rates results in four groups were as follows,(864.88±235.70)％,(1029.68％±268.40)％,(1169.76±104.15)％ and (1023.38±68.99)％.No significant difference was observed (P>0.05).Compression modulus values for each group were recorded as follows,(77.67±22.94)kPa,(132.67±21.83)kPa,(159.33±18.01)kPa and(301.33±76.43)kPa.The results indicated that compression modulus increased progressively with increasing specific gravity of nHA.Moreover,the compression modulus in the nHA group was significantly stronger than that of the SIS sponge group(P<0.05).The first three groups showed low cytotoxicity whereas the fourth one(SlS:nHA=l︰4)exhibited certain cytotoxicity due to its higher nHA content.Conclusion Adding nHA to the SIS sponge can significantly improve the strength of the scaffold material.The SIS︰nHA=1︰2 can be chosen as a suitable scaffold for bone tissue engineering.
Keywords：Small intestine submucosaNano-hydroxyapatiteBone tissue engineeringComposite scaffold
Publication Date：2024-07-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 193-199 )