Porphyromonas gingivalis promotes neuroninflammation by regulating the expression of NLRP3 pathway in microglia
LI Xue
ZHU Qing-yu
WANG Yan
QI Sheng-cai
Abstract：Objective To investigate the role and mechanism of Porphyromonas gingivalis(P.gingivalis)metabolites in promoting neuroinflammatory response by activating the NLRP3 inflammatome.Methods Microglia HMC3 cells was treated with P.gingivalis supernatant.CCK8,real-time fluorescent quantitative PCR,and Western blot were used to detect P.gingivalis supernatant's activation of inflammation in microglia HMC3 cells,its validation of inflammation activation and mitochondrial function verification.Results P.gingivalis supernatant,the cell viability of microglia HMC3 was inhibited(P＜0.05).The NLRP3 inflammatome of microglia HMC3 was significantly activated by 5%P.gingivalis supernant,and the apoptosis-associated spot-like protein(ASC),Caspase1 and IL-1β were increased(P＜0.05).The expression of mitochondrial function-related proteins and mitochondrial membrane potential decreased.After addition of NLRP3 inflammation inhibitor(MCC950),the CCK8 cell viability was significantly enhanced at 10nM MCC950,and apoptosis-associated spot-like protein(ASC),Caspase1 and inflammatory factor(IL-1β)were inhibited at 10nM MCC950(P＜0.05).Mitochondrial function was also restored.Conclusion P.gingivalis promotes microglial inflammatory response by activating NLRP3,which may be related to mitochondrial function.
Keywords：Porphyromonas gingivalisPeriodontitisNLRP3MicrogliaNeuroinflammation
Publication Date：2024-05-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 134-140 )