circRNA EPB41L2 promotes proliferation,migration and invasion of oral squamous cell cells by regulating miR-1270
HE Ping
CAO Yang
ZHANG Ting
Abstract：Objective The purpose of this study was to investigate the effect of circular RNA(circRNA)EPB41L2 on the proliferation,migration and invasion of oral squamous cell carcinoma(OSCC)cells by regulating the miR-1270/TFRC axis.circRNA EPB41L2 has been reported to be abnormally expressed in cancer,but its role in tumor growth and metastasis remains unclear.Method The expression levels of circRNA EPB41L2,miR-1270 and TFRC were analyzed by qRT-PCR.The proliferation,migration and invasion of OSCC cells were analyzed by clone formation and Transwell assay,respectively.The expression of epithelial-mesenchymal transition(EMT)markers was analyzed by Western blotting.Dual luciferase and RIP assays were performed to explore the interaction between circRNA EPB41L2,miR-1270 and TFRC.Result circRNA EPB41L2 was up-regulated in OSCC cells,and cell function experiments showed that circRNA EPB41L2 knockdown inhibited cell proliferation,migration,and invasion.Moreover,mechanism studies have shown that circRNA EPB41L2 can bind to miR-1270 and up-regulate its target gene TFRC,thereby promoting EMT of OSCC cells.Conclusion The results of this study demonstrated the role of circRNA EPB41L2 in OSCC tumorigenesis and metastasis,and circRNA EPB41L2 plays its role in promoting cancer through the miR-1270/TFRC axis in OSCC.
Keywords：circRNA-EPB41L2miR-1270TFRCOral squamous cell carcinomaproliferation.
Publication Date：2023-09-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 264-270 )