A clinical study on plasma miRNA-486-5p as a marker of recurrence of oral squamous cell carcinoma
WANG Xuan
YAN Yan
GAO San
SUN Zheng
Abstract：Objective:To screen and verify the potential of plasma miRNA-486-5p as a biomarker of for recurrence oforal squamous cell carcinoma.Methods:According to the inclusion and exclusion criteria,28 patients with oral squamous cell carcinoma and 21 healthy controls were selected.Plasma samples were collected 1 week before operation and 1 year after operation in the experimental group and once in the control group.8 cases of squamous cell carcinoma and 3 cases of control group were used to construct cDNA libra,.After high-throughput sequencing,the target miRNA was screened,and the remaining plasma samples were used to verify target miRNA.Results:The highest expression of miRNAs in plasma was miRNA-486-5p,accounting for 39.6％ of the total expression of miRNAs,and there was a differential expression between the groups.In the postoperative non-recurrent and normal control group,the expression level was higher than that in the preoperative group (P＜0.01,P＜0.05),but while there was no significant difference between the postoperative recurrence group and preoperative group(P＞0.05).Conclusion:The expression level of plasma miRNA-486-5p is related to the recurrence of oral squamous cell carcinoma and can be used as a candidate biomarker for monitoring the recurrence of tumor after tumor operation.
Keywords：plasma miRNAoral squamous cell carcinomahigh throughput sequencingrecurrencebiomarkers
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 142-146 )