Effects of new mineralized collagen membrane on biological behavior of human osteosarcoma cell line Saos-2
HAN Xue
WANG Chen
JIANG Hao
XU Yi-quan
Abstract：Objective:To investigate the biological effects of a new mineralized collagen membrane on Saos-2 cells in vitro.Methods:The Saos-2 cells were cultured on the mineralized collagen membrane and the morphology was observed by the light microscope.The extract liquid from mineralized collagen membrane and Bio-gide membrane was made.There were three groups:the blank control group,Bio-gide group and mineralized collagen membrane group.The cell proliferation and ALP activity of three groups were studied.The data were analyzed by SPSS 17.0 software.Results:Saos-2 cells on the mineralized collagen membrane readily attached to it and exhibited an elongated appearance.The MTT assay showed that the proliferation proceeded in three groups.There was no significant difference after 1,4,7 days culture among three groups in statistics.But the OD value of Saos-2 in control group were the higher than the other two groups after 10 days.The ALP activities after 7 days culture were different in the three groups.The ALP activity of mineralized collagen membrane group was the highest,and that of the blank control group was the lowest.The difference in expression of ALP among three groups was statistically significant.Conclusions:Studies in vitro revealed that the mineralized collagen membrane has good cell biocompatibility.
Keywords：collagen membranecell cultureSaos-2 cellscell proliferation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 71-74 )