High glucose and free fatty acid inhibit proliferation and induce apoptosis in osteoblast
WU Juan
QIU Qi-hong
YANG Ji-feng
LIANG Min
Abstract：Objective:To investigate the effect of the combination of high glucose and free fatty acid on apoptosis and proliferation in osteoblast.Methods:Osteoblasts were treated with high glucose and free fatty acid (D-glucose 20.0mM,PA 0.4mM,BSA 0.5％) for 0-48h.Phase-contrast microscope was used to observe morphologic alteration of cells.Proliferation was detected by CCK-8.Apoptosis was analysed by Annexin Ⅴ/PI double dye staining followed by flow cytometry analysis.Western blotting was applied to examine protein expression of caspase-3 and cleaved caspase-3.Results:Combination of high glucose and free fatty acid caused cell shrinkage,rounding as well as detachment after 24h.CCK-8 data showed that high glucose and free fatty acid inhibited cell proliferation in a time dependent manner compared to control group (F24h=24.489,P=0.001;F48h=272.440,P＜0.001).Flow cytometry results revealed that in the present of high glucose and free fatty acid,the apoptotic rate was 9.68％±3.33％ after 24h and increased to 12.89％±3.40％ after 36h compared to control group (1.96％±1.32％).Western Blotting indicated that healthy osteoblasts rarely expressed cleaved caspase-3.High glucose and free fatty acid increased cleaved caspase-3 protein expression by 2.4 times (0.349±0.068,F=9.603,P=0.016) and 3.2 times (0.468±0.136,F=9.603,P=0.001) after 24h and 36h respectively compared to control group (0.146±0.033).Conclusion:High glucose and free fatty acid time-dependently inhibit proliferation and increase apoptosis in osteoblast.
Keywords：high glucose and free fatty acidosteoblastproliferationapoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 65-70 )