The effect of molecular biological behaviorof Zirconiaon mouse periodontal ligament cells
HUANG Yu-xi
WANG Li-li
ZHANG Zhen-ting
LI Jing-rong
LIU Hong-liang
DONG Hai-tao
Abstract：Objective:To study the effect of molecular biological behavior of Zirconia on mouse periodontal ligament cells.Methods:PDLCs were cultured in vitro.Extracting solution ofZirconia,Pd-Ag alloy porcelain,and Ni-Cr alloy porcelain were prepared and used to process PDLCs,and then cell viability,percentages of cell cycle and expression levels of Caspase-3,Caspase-8,Caspase-9 were detected.Results:There was no significant difference in group of DMEM,group of Zirconia and group of Pd-Ag alloy porcelain in cell viability when co-cultured 24h,48h,72h later(P＞0.05).Cell viability of group of Ni-Cr alloy porcelain was lowest in all groups(P＜0.05).There was difference in group of Zirconia,group of Ni-Cr alloy porcelain and group of Pd-Ag alloy porcelain in early phase rate of apoptosis,late phase rate of apoptosis and totle rate of apoptosis(P＜ 0.05).There was difference between Zirconia and Pd-Ag alloy porcelain in index of Prl(P＜ 0.05).But there was no difference between Pd-Ag alloy porcelain and Ni-Cr alloy porcelain in index of Prl (P＞0.05).There were no difference in the value of 2-△△CT about Caspase-3 and Caspase-9 in group of Zirconia,group of Pd-Ag and group of DMEM (P＞0.05).The value of 2-△△T about Caspase-3 and Caspase-9 of Ni-Cr alloy porcelain was higher than that of DMEM (P＜ 0.05).There were no difference in the value of 2-△△CT about Caspase-8 in Zirconia andPd-Ag alloy porcelain(P＞ 0.05),and both lower than that of DMEM(P＜ 0.05).The value of 2-△△CT about Caspase-8 of Ni-Cr alloy porcelain was higher than that of Zirconia and Pd-Ag alloy porcelain(P＜0.05).Conclusion:Zirconia is superior to Pd-Ag alloy porcelain and Ni-Cr alloy porcelain in cell viability and anti-apoptosis.It has good histocompatibility and will not affect cell viability,cell cycle and expression of cell apoptosisralated genes.
Keywords：Zirconiamolecularbiological behaviorperiodontal ligament cellsapoptosisCaspase
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 12-17 )