The changes of Wnt/β-catenin signaling pathway in bone marrow mesenchymal stem cells under aging condition

LIU Na; ZHANG Xian-hua; LI Ying; ZHANG Wei; YANG Kun; GU Bin

doi:10.3969/j.issn.1672-2973.2018.01.002

Abstract：Objective:To investigate the changes of bone marrow mesenchymal stem cells (BMSCs) and the changes of Wnt/β-catenin signaling pathway under aging condtion.Methods:3-month-old and 18-month-old were used in our study,the femur density were detect by dual energy X-ray.We isolated BMSCs from bone marrow and then the SA-β-Gal expression was analyzed to assess the cell senescence.The expression of telomerase reverse transcriptase (TERT) mRNA was detected by real-time quantitative PCR.The secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was detected by immuno-enzyme-linked immunosorbent assay.Western blot was used to detect the expression of β-catenin in the cytoplasm and nucleus.Real-time quantitative PCR was used to detect the expression of LEF-1 and TCF-4.Results:The femur density in the 18-month-old group was significantly lower than that in the 3-month-old group.The expression of SA-β-Gal positive cells increased in BMSCs (18-month-old group) compared with 3-month-old group,however,the expression of TERT was significantly decreased in BMSCs (18-month-old group).The secretion of TNF-α and IL-6 in BMSCs was significantly increased in 18-month-old group than in 3-month-old group.The expression of β-catenin in the nucleus and cytoplasm of BMSCs in 18-month-old group was higher than that in 3-month-old BMSCs group.The expression level of group LEF-1 in the 18-month-old group was 1.6 times higher than that of the 3-month-old group,and the gene expression level of TCF-4 also increased 3.12 times than that of the 3-month-old group.Conclusion:With the increase of age,the content of inflammatory cytokines secreted by BMSCs were increased,which led to the activation of Wnt/β-catenin signaling pathway and then affect the biological function of BMSCs.