Effect of tantalum-substituted hydroxyapatite (HA) coating on the attachment and proliferative activity of mesenchymal stem cells
LU Rong-jian
HE Hui-xia
E Ling-ling
ZHANG Gui-lan
NING Cheng-yun
LIU Hong-chen
Abstract：Objective:To study the effect of tantalum-substituted HA coating on the attachment and proliferation of mesenchymal stem cells,which provide experimental basis for the design and application of dental implant coating materials.Methods:Tantalum-substituted HA coatings were prepared on titanium using atmospheric plasma spraying.The surface topography,composition,roughness and contact angle of tantalum-incorporated HA coating were characterized.Then the adhesion,spread and proliferation of rat bone mesenchymal stem cells were investigated by cells counting,electron microscopic observation and MTT assay.Results:Tantalum-substituted HA coatings have a porous structure,with higher surface roughness and hydrophilicity.The addition of tantalum dramatically increased the bonding strength of HA coating to titanium substrate.There are more cells attaching on tantalum-substituted HA coatings compared to titanium,which spread well and have a higher proliferation activity.Conclusion:Ta-substituted HA coating prepared by atmospheric plasma spraying has good cytocompatibility,and could promote the attachment and proliferative activity of mesenchymal stem cells.
Keywords：tantalumhydroxyapatite coatingmesenchymal stem cellsattachmentproliferative activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 321-327 )