Role of different scale structures of titanium surface on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells
ZHOU Min
WU Dong-hui
WU Ya-lin
PANG Jing-wen
ZHUANG Xiu-mei
Abstract：Objective:To investigate the effect of titanium surface scale structures on the cell viability and osteogenic differentiation in rat bone marrow mesenchymal stem cells (rBMSCs).Methods:Alkali heat treatment at high and low temperatures were utilized to nano-modify sandblasted titanium with microtopographical surfaces,compared with microtopographical surfaces of conventional sandblast-acid etching and smooth surface in titanium control.rBMSCs were seeding on these four titanium discs,and the cell viability was detected at 1,3,5 and 7 days by CCK8 assay.Total protein values and alkaline phosphatase (ALP) activity were examined at 7 and 14 days.qRT-PCR was used to detect mRNA expression of ALP,collagen-Ⅰ (COL1) and runt related transcription factor 2 (RUNX2) at 7 days.The date was statistically analyzed with SPSS 13.0 software package.Results:Compared with Ti control and sandblast-acid etching surfaces,rBMSCs on the micro-nanotopographical surface discs in alkali heat treatment at high temperatures gourp and low temperatures group exhibited enhanced cell viability,increased total protein values,up-regulated ALP activity and overexpression of ALP,COL1 and RUNX2 levels.Conclusion:Micro-nanotopographical surface of titanium implant promotes cell viability and osteogenic differentiation ofrBMSCs.
Keywords：Rat bone marrow mesenchymal stem cellsScale structures of titanium surfaceOsteogenic differentiationMicro-nanotopographical surface
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 261-265 )