Effects of insulin, estrogen and culture time on the proliferation of newborn rat mandibular osteoblasts
E Ling-ling
XU Wen-huan
QIAO Peng-yan
XING He-lin
WANG Xing
FENG Lin
LV Yan
WANG Dong-sheng
LIU Hong-chen
Abstract：Objective:To investigate the effect of insulin (In),estrogen (E2) and culture time on the proliferation of newborn rat mandibular osteoblasts (NRMOBs).Methods:The NRMOBs were cultured in vitro.The effect of insulin (6ug/mL) and/or estrogen (10-9 moUL) and/or culture time on NRMOBs was investigated.The NRMOBs proliferation was examined after 1、3、5、7、9 days of culture using Methylthiazol Tetrazolium (MTT).Results:The NRMOBs positively expressed vimentin and osteocalcin,negatively expressed keratin,secreted alkaline phosphatase (ALP),and produced calcium nodules.When NRMOBs were cultured in the media with 5.5mM glucose,estrogen significantly decreased the cells proliferation at 1 day of culture,insulin significantly increased the cells proliferation at 7,9 day of culture.Compared to 5.5mM+E2,5.5mM+Insulin significantly increased NRMOBs proliferation at 7,9 day of culture.The combination of insulin andestrogen had no effect on the proliferation of NRMOBs at every time point.Conclusion:This study suggests that insulin or estrogen alone affects NRMOBs proliferation in different culture time.The combination of insulin and estrogen can adjust the effect of insulin or estrogen alone on NRMOBs proliferation.
Keywords：osteoblastsinsulinestrogenmandibularrat
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 257-260,269 )