Research differences of biological functions between stem cells from human exfoliated deciduous teeth and dental pulp stem cells
PANG Zhen-zhen
WANG Liang
GAO Lei
Liu Qin-zan
LI Ye
Abstract：Objective:Research differences of biological functions between stem cells from human exfoliated deciduous teeth and dental pulp stem cells from different angles,to explore the influence of bone reconstruction.Methods:SHED and DPSCs were isolated,purified and cultured in vitro by limiting diluction,and oil red O staining at 14 days of adipogenic induction,alizarin red staining at 21 days of osteogenic induction;detect IL-6 and TNF-α secretion level by Elisa method;ALP staining at 7 days of two kinds of stem cells in conventional cultivation and osteogenic induced,and detect Runx2、RANKL and OPG gene expression by real-time PCR.Results:Both of SHED and DPSCs have abilty to adipogenic and osteogenic differentiation;conforms to the characteristics of the stem cells.SHED expression of IL-6 and TNF-α were higher than that of DPSCs(P＜ 0.05);Both kinds of stem cells express Runx2OPG and RANKL after osteoinductive differentiation,but the expression of SHED obviously higher than that of DPSCs(P＜0.05).Conclusion:Compared with DPSCs,SHED has not only the ability of osteogenic differentiation but also an osteoclast capacity,remind that SHED may participate in the bone reconstruction.
Keywords：human exfoliated deciduous dental pulp stem cellsdental pulp stem cellsTL-6Runx2
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 204-208 )