The preliminary research on geriatric dental pulp stem cells from an 88-year-old man
QIAO Peng-yan
LIU Hong-chen
Abstract：Objective:To isolate and obtain dental pulp stem cells (DPSCs) from the oldest-old and explore their biological functions.Methods:The dental pulp cells from an 88-year-old male patient's upper third molar were isolated using the enzymatic digestion of pulp tissue method,cultured,passaged and analyzed by flow cytometry.Cell morphology,proliferation and differentiation ability were observed in vitro.Results:It is the first to successfully isolated,cultured and passaged the geriatric dental pulp stem cells(GDPSCs).The morphology of the cells were spindle or exhibited several pseudopods under inverted phase contrast microscope and were positive for human multipotent mesenchymal stromal cells (MSCs) surface markers CD73,CD90,and CD 105,but negative for CD 14,CD 19,CD34,CD45 and HLA-DR.These cells also exhibited good self-renewal ability and multilineage differentiation potential.Conclusion:DPSCs can be isolated from the pulp tissues of the oldest-old and present well proliferation and differentiation potentials.
Keywords：dental pulp stem cellsregenerative medicinetissue engineeringoldest-oldgeriatric dental pulp stem cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 193-197 )