Effect of hypoxic condition on proliferation and apoptosis of human periodontal ligament cells via hypoxia inducible factor-1α
PANG Jing-wen
WU Ya-lin
XU Ting
ZHUANG Xiu-mei
Abstract：Objective:To investigate the effect of hypoxia on proliferation and apoptosis in human periodontal ligament cells (PDLCs),and the role of HIF-1α in this process.Methods:PDLCs were cultured under hypoxia (1％ O2) or normoxia.MTT was used to detect cell viability of PDLCs treat with or without hypoxia.Effect ofhypoxia on apoptosis of PDLCs was detected by Annexin V-APC on a flow cytometer.Western blot and qRT-PCR were used to detect expression of HIF-1 α at protein and mRNA level respectively.HIF-1 (HIF1) was transfected into PDLCs by small interfering RNA.HIF-hα,cell viability,as well as apoptosis,were furthered detected in PDLCs.Results:In the hypoxic environment,the activity of PDLCs was significantly inhibited after 48 h of culture.The level of apoptosis was increased,and the levels of HIF-1 protein and mRNA were significantly increased After knocking down of HIF-1α with siRNA,HIF-1α was significantly downregulated in PDLCs under hypoxia,as well as increased cell viability but decreased apoptosis.Conclusion:Hypoxia inhibits proliferation and promotes apoptosis of PDLCs via upregulated HIF-1 α.
Keywords：Periodontal ligament cellsHypoxiaProliferation and apoptosisHypoxia inducible factor-1α
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 170-174 )