The periodontal ligament stem cells and biological characteristics of human dental pulp stem cells and phenotype
CAI Hong-zhen
HE Hui-xia
WANG Fei-xiang
ZHANG Shao-qing
Abstract：Objective:We aim to compare the biological characteristics and phenotypic after cultivating and separating of the human Periodontal ligament stem cells (hPDLSCs) and human Dental pulp stem cells (hDPSCs).Methods:HPDLSCs and hDPSCs were respectively separated and amplificated ~om the original generation of cultures by magnetic-activated cell selection system (MACSS).Inverted phase contrast microscope was employed to observe the morphology.What is more,we make use of CCK-8 method to check and draw up the growth curves.Moreover,the expression of stem cell surface markers were analysized by flow cytometry instrument (FCM).Finally,we analyse and compare the colony formation ratio (CFR).Results:Fusiform or triangle,the morphology of hPDLSCs and hDPSCs is similar.The growth curvesare "S" shape.The expression ratio of STRO-1 in hPDLSCs and hDPSCs is respectively 15.88± 0.48％ and 11.86± 0.43％.There is no significant difference between the two cells.But the hDPSCs colony formation rate (4.31 ± 0.08％) is significantly higher than that of the hPDLSCs (2.68± 0.06％)(P＜0.05).Both of the two stem cells are positive for the CD29,CD44,CD90 and CD105 which are characterized by the employed to distinguish hPDLSCs and hDPSCs,and we can conclude that the separated stem cells share similar morphology.HPDLSCs and hDPSCs that can refresh and differentiate multi-directionally are characterized by stem cells due to the expression of stem cell markers.But the hDPSCs owns a significantly stronger colony formation rate than that ofhPDLSCs,then we can make a conclusion that hDPSCs is a promising stem cells resource.
Keywords：human periodontal ligament stem cellshuman dental pulp stem cellphenotype
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 91-96 )