Effects of lipopolysaccharides on the proliferation and IL-8 secretion of periodontal ligament fibroblasts

FU Chang-sheng; LIU Rong-sen; LUO Yun; OU Long; LI Ying-chao; ZHANG Xian-hua; WANG Jun; DU Yan

doi:10.3969/j.issn.1672-2973.2017.02.005

Abstract：Objective:To observe the effects of different concentrations of lipopolysaccharides on the proliferation of periodontal ligament fibroblasts and the secretion of IL-8.Methods:The experiments were divided into seven groups,human periodontal ligament fibroblasts (hPDLFs) group,PDLFs + 10μg/ml basic fibroblast growth factor (BFGF) group,1 μg/ml LPS group,10μg/ml LPS group,100μg/ml LPS group,200μg/ml LPS group,500μg/ml LPS group.The cells were cultured and MTT assay was used to observe the cell proliferation.The cell supernatant after cultured for 24h and 96h was collected of IL-8 ELISA.Results:Synthesis and release of IL-8:Compared with periodontal ligament fibroblasts group,there were no statistically significant differences in 1 μg/ml LPS,10μg/ml LPS and 1 00 μg/ml LPS groups after cultured for 24h;significant statistical differences were detected in 200μ g/ml LPS,500μg/ml LPS groups.Compared with periodontal ligament fibroblasts group,there were no statistically significant differences in 1 μg/ml LPS and l0μg/ml LPS group after cultured for 96h;significant statistical differences were detected in l00μg/ml LPS,200μg/ml LPS,500μg/ml LPS groups,there was no significant difference between 100μg/ml LPS and 200μg/ml LPS group.Proliferation of human periodontal ligament fibroblasts:there were no statistically significant differences between 1 μg/ml LPS,100 μg/ml LPS and control groups.The promotion of the proliferation of periodontal ligament fibroblasts was observed in 10 μg/ml LPS group.200μg/ml LPS,500μg/ml LPS significantly inhibited the proliferation of PDLFs.Conclusion:Different concentrations of lipopolysaccharides can regulate the proliferation of periodontal ligament fibroblasts and the synthesis and release ofIL-8.