Effects of glucose on mandibular marrow stromal cells differentiation into osteoblastic lineages in rats
LI Ying
WANG Su-yu
XIAO Yi-han
WANG Jun-cheng
Abstract：Objective:To observe the effects of different concentration of glucose on osteogenic differentiation of bone marrow stromal cells (BMSCs) obtained from rats' mandibular.Methods:The in vitro technique of cell culture was employed to harvest,purify and cultivate BMSCs from the mandibular of rats.Flow cytometry analysis of cell phenotypes and osteogenic differentiation were performed.The passage 4 cells were incubated with osteogenic DMEM containing 5.5,16.5,25,and 35 mM glucose,respectively.The cells were then stained with 2％ Alizarin red S and the Alizarin red S-stained nodules were observed microscopically and quantified.ALP staining and ALP activity assay were performed.The calcium and phosphonium content were assayed.Then the expression of bone morphogenetic protein-2 (BMP-2) was analysed by western blot and the expression of RUNX2 mRNA was analysed by real-time PCR.The data were subjected to one-way ANOVA using SPSS 11.0.Results:With the increasing of glucose concentrations,the number of red calcium nodules obviously decreased,ALP activity was inhibited,and the calcium and phosphonium content in medium were decreasd.The difference was significant (P＜0.05).Furthermore,BMP-2 levels and RUNX2 mRNA expression were decreased (P＜0.05).Conclusions:In high glucose microenvironment,osteogenic capacity of marrow stromal cells from rat mandibular was inhibited,and the BMP signaling pathway may be involved in this process.
Keywords：High glucose enviromentBone marrow stromal cellsosteogenic differentiation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 257-262 )