Effects on osteoblast morphology and proliferation in estrogen deficiency rats
LI Chuan-jie
WANG Jun-cheng
LIU Na
WEI Ze-ning
LU Rong-jian
E Ling-ling
LI Ya-nan
Abstract：Objective: T o observe the effects on osteoblast proliferation and the cell m orphological changes in ovariectom ized rats.The osteoblastdifferentiation ability ofinduced osteoporosis rats w hich w ere estrogen deficiency w as analysed.Methods:3-m onth old unm ated fem ale SD rats w ere divided random ly into tw o groups:sham operated (G roup A) and ovariectom ized (G roup B) . T he O V X group underw ent bilateral oophorectom y through a back incision, w hile the sham group rats only received partial resection of fatty tissue on the back. T o observe the effects on bone m ass in ovariectom ized rats w hich had a low level of estrogen, w e detected the level of estrogen 1.5 m onths after operation by electrochem ilum inescence ECL(ECLIA ), w eighed the w eight changes regularly and m easured bone density changes of lum bar spine, bilateral fem ur of rats by dual energy absorptiom etry before operation and 1.5 m onths after operation. T he calvaria bone ofthe rats w ere harvested at1.5 m onths to culture osteoblasts w ith tissue block m ethod and cellcounting w as performed.ThenthedynamicchangesofmorphologicaldifferencesandthechangesinbonecellproliferationbyM TSwere investigated.Results:1.B ody condition:the body w eights of rats in ovariectom ized group w ere increased significantly (P<0.01) com pared to the sham group ,w hile the bone density of ovariectom ized rats decreased statistically com pared w ith the sham group (P<0.01). 2.M orphological observation:T he ovariectom ized rat cell craw ling speed w as slow . T he osteoblast proliferation rate w as significantly slow er,w hile the proliferation cycle w as relatively prolonged and the osteoblasts num ber w aslessthan norm aloverthe sam e period.Ittook the P1 cellsin G roup A 8 daysto reach 80% confluence,w hile in G roup B about4days.M uchmoreintracellularblackparticlesandthecavitationphenomenonwereobservedinP1osteoblastin the O V X group than in the SH A M group, indicating slow er cell proliferation and irregular cell confluence. O steoblasts senescence in ovariectom ized group (about 60% ) w as significantly m ore than in the sham group. 3.C ell proliferation:, the proliferation ability of the ovariectom ized rats osteoblasts in group B w as significantly low er than that in group A . O steoblastgrew slow ly and soon entered the platform period ofproliferation.Conclusion:A ftercastration,the body w eight of rats w as increased and the bone m ineral density w as decreased significantly. O steoblast m orphology w as changed in O V X rats, m oreover, the cell proliferation ability w as reduced, and the cavitation phenom enon w as obvious., indicating accelerated aging and the increased aging cellnum berratio.
Keywords：postm enopausalosteoporosiscastration operationosteoblastcellm orphologycellproliferation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 132-137 )