Histological changes in atrophy of the rat parotid gland after duct-ligation
LU Hao
ZHANG Ci-tong
LIU Kang
Sun Bin
Liu Qi-lin
ZHANG Wei
Abstract：Objective:To investigate the histological changes in atrophy of the rat parotid gland after duct-ligation. Methods:The excretory ducts were double-ligated with silk suture unilaterally to induce atrophy of the parotid glands of Wistar rats for either 0,1,3 ,7,14 or 30 days respectively. The gland were harvested and examined with AB-PAS and Masson staining. Caspase3 and Ki-67 immunohistochemistries were detected.Results:After duct ligation, the vast majority of acinar cells and the zymogen granules were vanished identified by AB-PAS staining accompanied by increased collagenous fibers. Caspase3 and Ki-67 positive cells were found occasionally in the sham operation group, but were extensively found during the atrophy of parotid glands. The expressions of Caspase3 and Ki-67 positive cells were statistically different between the two groups(P<0.05).Conclusion:During atrophy of the parotid gland, most acinar cells and zymogen granules vanished accompanied by increased collagenous fibers. The salivary fistula could be avoided by ligate the stensen’s duct in surgeries of parotid region.
Keywords：Caspase3Ki-67ApoptosisFibrosisParotid gland
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 85-87 )