Osteogenic diffetentiation ability of mandibular marrow stromal cells from type 2 diabetic rats in vitro
WANG Jun-cheng
WANG Bin
E Ling-ling
GUO Jing
LIU Hong-chen
Abstract：0bjective:To observe osteogenic differentiation ability of bone marrow stromal cells (BMSC) obtained from type 2 diabetic rats mandibles.Methods:The invitrotechnique of cell culture was employed to harvest, purify and cultivate BMSCs from the mandibles of type 2 diabetic(GK, test group) and normal rats (Wistar,control group) . Flow cytometry analyses of cell phenotype and osteogenic/adipogenic differentiation were performed. The passage 3 cells were incubated with osteogenic DMEM. The cells were then stained with 2% Alizarin red S. The Alizarin red S-stained nodules were observed microscopically and quantified. ALP staining and ALP activity assay were performed. The calcium content and phosphonium content were evaluated. The expression ofRUNX2mRNA was analysed by real-time PCR. The data were subjected tot-testusing SPSS software.Results:BMSCs were obtained from the mandibles of type 2 diabetic rats successfully. Compared with BMSCs derived from normal rats, the number of red calcium nodules in the test group were obviously decreased, ALP activity was inhibited and the contents of calcium and phosphonium in medium were also decreased. The difference was statistically significant (P<0.05). Furthermore,RUNX2mRNA expression was decreased (P<0.05).Conclusion:The osteogenic differentiation of type 2 diabetic rats mandibular marrow stromal cells was inhibited significantly. Morever,the decreased osteogenic differentiation ability cannot be restored even they were cultured in normal DMEM.
Keywords：Type 2 diabetes mellitusratsbone marrow stromal cellsosteogenic differentiation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 70-74 )