Notch signal pathway is involved in BMP-6-induced proliferation and odontoblastic differentiation of human dental pulp cells
ZHENG Qiong-dan
XING Quan
LIAO Wei-li
LI Mei-lin
CAI Hua-xiong
Abstract：Objective:To investigate the involvement of the Notch pathway in bone morphogenetic protein-6(BMP-6)-induced proliferation and odontoblastic differentiation of human dental pulp cells (hDPCs) in vitro.Methods:The hDPCs were cultured by the methods of tissue explants digested with collagenase. Cells from the third-fifth passages were treated with BMP-6 or/and DAPT, aγ-secretase inhibitor. The proliferation and ALPase activity of hDPCs were tested by using CCK-8 assay and ALP kit, and mineralization nodules were observed by Alizarin Red Staining.Results:CCK8 and ALP results showed that BMP-6 induced proliferation and odontoblastic differentiation of hDPCs, while DAPT palyed an opposite role. Treatment with a combination of BMP-6 and DAPT reversed the increase of both proliferation and odontoblastic differentiation induced by BMP-6 alone. Mineralization was significantly increased in response to BMP-6 treatment, while DAPT attenuated mineralization induced by BMP-6 in hDPCs.Conclusion:BMP-6 can promote the proliferation and odontoblastic differentiation of hDPCs, and suppressed the Notch pathway and can significantly attenuate the effects of both proliferation and odontoblastic differentiation of hDPCs induced by BMP-6.
Keywords：Notch pathwayBMP-6human dental pulp cellsproliferationodontoblastic differentiation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 65-69 )