Biocompatibility of injectable nHAC/CSH with dog peripheral blood stromal cells
HAN Xue
ZHOU Wei
WANG Dong-sheng
E Ling-ling
WU Xia
LIU Hong-chen
Abstract：Objective:The aim of the study was to assess the cell biocompatibility of injectable nano-hydroxyapatite/colla-gen/calcium sulphate hemihydrate (nHAC/CSH) bone cement.Methods:Dog peripheral blood stromal cells (dPBSCs) were isolated from peripheral blood of healthy adult dogs. An in vitro study on the cytocompatability of nHAC/CSH was con-ducted. The dBMPCs morphology, proliferation and apoptosis assays were conducted using the direct contact and extraction method by microscopic observation, MTT analysis and flow cytometric method.Results:The MTT assay showed that pro-liferation proceeded in two groups. There was a spontaneous apoptosis of dBMPCs, with similar apoptosis rates on Day 3 in both the control culture and the extract group. Cells grew with nHAC/CSH cement readily attached to it and exhibited an e-longated appearance.Conclusion:Studies in vitro revealed that the nHAC/CSH cement has good cell biocompatibility.
Keywords：bone tissue engineeringinjectablecell culturecell proliferationcell apoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 15-18 )