Effects of Endothelin-1 on the alkaline phosphatase activity and the production of osteocalcin of periodontal ligament cells
LIANG Li
ZHOU Wei
YU Ji-feng
E Ling-ling
LIU Hong-chen
Abstract：Objective:To investigate how endothelin-1 affects the alkaline phosphatase( ALP) activity and the production of osteocalcin(OCN) of periodontal ligament (PDL) cells.Methods:The PDL cells were stimulated with endothelin-1 (1,10, 100nM) for 48 hours. The untreated hPDL cells served as normal control. ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit.Results:The ALP activity and the production of OCN of PDL cells were reduced by ET-1 stimulation in dose-dependent manner.Conclusion:These results indicated that ET-1 could reduce the ALP activity and the production of OCN of PDL cells.
Keywords：endothelin-1periodontal ligament cellsalkaline phosphatase activityosteocalcin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 12-14 )