Study on differentiation of adipose derived stem cells into odontoblast-like cells induced by rat apical bud
SUN Ya-juan
LI Hua
LIU Na
ZHANG Bo
GU Bin
Abstract：Objective:To study the effect of rat apical bud in differentiation of ADSC into odontoblast-like cells in vitro and in vivo.Methods:Rat ADSCs and apical bud were isolated and then ADSCs were cultured with conditioned medium (from rat incisor apical bud cells). After 7 days of induction, the gene expression of DMP-1 and DSPP were analyzed by real time PCR. ADSCs mixed with prepared apical bud and the reconstituted cell mass were implanted under rat renal capsules for up to 8 weeks, analyzed with histological and immunohistochemical evaluation.Results:Compared with the control group, apical bud conditioned medium treatment significantly induced the mRNA expression level of DMP-1 and DSPP in ADSCs. Histological examination of the product of reconstituted cells mass of apical bud epithelium and ADSCs showed positive results. Hematoxylin and eosin (H&E) staining showed tube-like dentin and bone-like dentin. Masson's trichrome staining showed green dentin-like structure. Immunohistochemidtry of CK14 and DSPP were both positive.Conclusion:Induced by apical bud epithelium conditioned medium, rat ADSCs can differentiate into odontoblast-like cells in vitro. Moreover,reconstituted cells mass of apical bud epithelium and ADSCs can construct dentin-like structure in vivo.
Keywords：apical bud epitheliumadipose derived stem cellodontoblastdifferentiate
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 7-11 )