Fabrication of bovine serum albumin/calcium phosphate bioactive coating on porous titanium scaffolds
ZHU Xiao-jing
YU Peng
NING Cheng-yun
WANG Yan
Abstract：Objectives: To fabricate apatite coating containing bovine serum albumin by co-deposition method on the surface of porous titanium scaffolds, and evaluate effects of incorporation of bovine serum albumin (BSA) on the morphology and chemical composition of the bioactive coating. Methods:The porous titanium scaffolds were prepared by using selective laser melting method. After alkali treatment, the porous titanium scaffolds were immersed into simulated body fluid for 4days for pre-calcification, subsequently two groups scaffolds were soaked in saturated calcium phosphate solution without or with containing BSA concentration of 10 μg/mL for 2 days. The topography of the specimen was observed by scanning electron microscopy (SEM). Chemical structure and phase composition of coatings were detected by Fourier infrared spectroscopy (FTIR) analysis and X-ray diffraction (XRD) respectively. Results: the addition of bovine serum albumin reduced crystal size of calcium phosphate, changing from micron grade structure to nanoscale;and coating without BSA was composed of hydroxyapatite and octocalcium phosphate, while BSA/calcium phosphate coatings contained hydroxyapatite. Conclusion: We successfully fabricated hybrid coating of BSA/calcium phosphate coatings on porous titanium scaffolds, and crystal size of coating reduced with the addition of BSA .
Keywords：selective laser claddingporous titanium scaffoldsbovine serum albuminsaturated calcium phosphate solutionco-depositioncalcium phosphate coating
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 140-143,148 )