Expression of the zinc-finger E-box binding homeobox 2 in human dental pulp tissue and cells
CHEN Li-hong
SHU Shan
WEI Xi
Abstract：Objective:To investigate the expression of zinc-finger E-box binding homeobox 2 in human dental pulp tissue and cells. Methods: Using fluorescence in situ hybridization (FISH) to test and verify the expression of ZEB2 in nomal dental pulp tissue. The expression levels of ZEB2 in normal hDPCs and hDPCs stimulated by TGF-β1 were detected by real-time fluorescentquantitative PCR (RT-qPCR) and FISH. Results: FISH array of pulp tissue showed that ZEB2 was mainly expressed in odontoblasts. The expression of ZEB2 was weakly positive in the nucleus and cytoplasm of normal hDPCs. TGF-β1 can promote the expression of ZEB2. The expression of ZEB2 was up-regulated obviously when hDPCs were stimulated by 5ng/ml TGF-β1. RT-PCR showed that ZEB2 mRNA tending to increase from 0h to 24h. There was a down trend a over time (P<0.05). After stimulated by TGF- β1 for 24h, the expression of ZEB2 was strong positive, especially in nuclear. Conclusion:ZEB2 was mainly expressed in odontoblasts in normal dental pulp tissue. The expression level of ZEB2 was positively correlated with TGF-β1 which suggested that ZEB2 involved in TGF-β1 signaling pathway and may play an role of regulation in the process of hDPCs differentiated into odontoblast.
Keywords：zinc-finger E-box binding homeobox 2/Smad-interacting protein1TGF-β1Human dental pulp cellsodontoblast
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 134-139 )