Effects of GSK3β on Osteogenic differentiation of human Periodontal Ligament Stem Cells in Inflammatory Microenvironment
LIU Na
JIANG Gui-xin
ZHANG Bo
GU Bin
LIU Hong-chen
Abstract：Objective:To investigate the osteogenic differentiation potential and the role of GSK3βof human periodontal ligament stem cells in inflammatory microenvironment. Methods: Periodontal ligament stem cells were obtained from human healthy individuals and patients with periodontitis (H-PDLSCs, P-PDLSCs). P-PDLSCs and H-PDLSCs were cultured in osteogenic medium for 7 days. We tested the mRNA expression of Runx2、 ALP、 and OCN by Real time PCR, protein expression of p-GSK3βand GSK3βwere examined by Western Blot. Stimulated PDLSCs whih LiCl in osteogenic induction process, bone formation ability was tested by ALP staining and RealTime PCR. Results: The osteogenic differentiation potential of P-PDLSCs was significantly reduced than the H-PDLSCs.We found more strikingly enhanced p-GSK3βP-PDLSCs than H-PDLSCs. After osteogenic differentiation, the expressions of p-GSK3βwas down-regulated, but the total GSK3βexpression level was up-regulated in both two types of PDLSCs. Both the ALP staining and mRNA expression levels of Runx2 and OCN showed that the osteogenic differentiation of PDLSCs decreased significantly after LiCl stimulated. Conclusion:The phosphorylation of GSK3βcan affect the Wnt signaling pathway, which mediated the impaired osteogenic differentiation of PDLSCs in inflammatory microenvironments.
Keywords：periodontal ligament stem cellsGSK3βinflammationOsteogenic differentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 129-133,158 )