Effects of LIMK2 RNA interference on the mechanosensitivity of COX-2 gene in osteoblasts
FU qiang
YANG Zhi
WU Chang-jing
CHEN Xiao-dan
TAN Shu-yi
Abstract：Objective: To study the effect of cytoskelet on reorganization in hibition with RNA interference on the mechanosensitivity of COX -2 gene in osteoblasts induced by fluid shear stress.Methods: RNA interference against LIMK2 gene was used to inhibit the cytoskeleton reorganization of BALB/cmouse prim ary osteoblasts.Osteoblasts were loaded by fluid shear stress at12 dyne/cm 2 or unloaded.The nested real-time PCR and immun of luorescence were performed to detect the expression levels of COX-2m RNA and protein, respectively. Result: Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene increased the expression of COX -2 gene induced by fluid shear stress.Conclusion: Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene could enhance the mechanosensitivity of COX -2 gene induced by fluid shearstress.
Keywords：OsteoblastCytoskeletonFluid Shear StressCOX-2 geneMechanosensitivity
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 325-329 )