In vitro modeling of interaction between Schwann cells from auditory nerve myelin and macrophages
ZHANG Si
LIU Ke
GONG Shusheng
Abstract：Objective To establish an in vitro model to study the interaction between induced M1-type macrophages and auditory nerve myelin Schwann cells.Methods Cochlear tissues from 7-day-old C57BL/6J mice were selected,and nerve fragments were digested to produce a mixed culture of Schwann cells and fibroblasts.Purified Schwann cells were obtained using differential adhesion and cytosine arabinoside treatment.Mouse bone marrow monocytes were polarized into M1 macrophages using macrophage colony-stimulating factor and lipopolysaccharide.The purified Schwann cells were then co-cultured with the induced M1 macrophages at a 1:1 cell ratio.Analysis was performed using immunofluorescence staining,cell counting,and enzyme-linked immunosorbent assay.Results The results showed a significant decrease in the number of Schwann cells on the third day after co-culture compared to the first day(P<0.001).There was a statistically significant difference in the secretion levels of brain-derived neurotrophic factor in the supernatant between the pure Schwann cell culture group and the co-culture group(P<0.001).Conclusions This study successfully established,a co-culture model of auditory nerve myelin Schwann cells and macrophages.This method holds significant value for in-depth research on the mechanism of macrophage influence on auditory nerve myelin Schwann cells and provides a foundation for understanding the immune response in the inner ear.
Keywords：myelin auditory nervecochlear macrophagesSchwann cellsneurotrophic factorco-culture model
Publication Date：2026-02-20
Online Publishing Date：2026-03-13(First online date of this platform, not the publication date of the document)
Pages：4( 137-140 )