Metabolic Characteristics of Blood Markers of Sudden Deafness Based on Untargeted Metabolomics
YU Wei
QIN Zeping
ZHU Xianbai
TAN Guojing
ZOU Can
DENG Anchun
Abstract：Objective To characterize metabolic profiles of potential biomarkers of sudden deafness by untargeted metabolomics.Methods Patients admitted to the Department of Otorhinolaryngology Head and Neck Surgery,the Second Affiliated Hospital of Army Medical University,between June 2022 and January 2023(n=30)were recruited,including 15 patients diagnosed with sudden sensorineural hearing loss(SSNHL)and 15 patients with simple deviated nasal septum and no hearing impairment.Venous blood samples were collected and subjected to untargeted metabolomics analysis using Liquid Chromatography-mass Spectrometry(LC-MS).Supervised Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA)was employed to identify significantly differential metabolites(variable importance in projection[VIP]>1,P<0.05),followed by pathway enrichment analysis.Results A total of 599 metabolites were identified in positive ion mode and 315 metabolites in negative ion mode.Quality control(QC)sample correlation analysis revealed R²values all approaching 1.OPLS-DA results indicated significant intergroup differences in metabolites.In positive ion mode,the OPLS-DA model demonstrated an R2Y of 0.974 and a Q2of 0.717,leading to the identification of 103 differential metabolites.In negative ion mode,the model showed an R2Y of 0.996 and a Q2of 0.571,with 65 differential metabolites identified.Enrichment analysis revealed significant clustering in 8 metabolic pathways.Pathway mapping analysis demonstrated elevated levels of phosphatidylcholine,O-acetylcholine,L-histidine,N-methylhistamine,phenylalanine,citric acid,and linoleic acid in the sudden deafness group compared to controls.Conversely,17α-hydroxyprogesterone,cortisol,androst-4-ene-3,17-dione,androgen,and 7-methylxanthine were found at lower concentrations in the sudden deafness group.Conclusion Lipid metabolism and amino acid metabolism may constitute principal underlying pathways involved in the metabolic dysregulation associated with SSNHL.The increase in the level of its metabolites may be one of the causes of sudden deafness.
Keywords：sudden deafnessuntargeted metabolomicsmetabolic markers
Publication Date：2025-10-20
Online Publishing Date：2025-10-17(First online date of this platform, not the publication date of the document)
Pages：8( 830-837 )