m6A Methylation and Transcriptome in Congenital Microtia
DU Yuling
MA Jing
GAO Yingqin
CHEN Quandong
XIAO Yang
LI Xia
ZHANG Hengying
LIN Ken
Abstract：Objective To understand modification of mRNA N6-methyladenosine(m6A)in congenital microtia.Methods Methylated RNA immunoprecipitation sequencing(MeRIP-seq)was used to compare m6A methylation in cartilage in microtia and normal ears.RNA sequencing(RNA-seq)was used to mine differentially expressed mRNAs.Through joint bioinformatics analysis using MeRIPseq and RNA-seq data,protein-protein interaction(PPI)networks were constructed,hub genes identified,and miRNA-target gene-IF regulatory networks constructed.Results A total of 17 076 differentially methylated m6A sites(DMMSs)and 9552 differentially methylated m6A genes(DMMGs)were identified by MeRIP-seq.RNA-seq revealed 1842 up-regulated genes and 3024 down-regulated genes.Combined analysis of MeRIP-seq and RNAseq data identified 96 differentially methylated and expressed genes.PPI networks identified 23 m6A related diagnostic genes,including EMX2,PPFIA2,HP and CDH20 that were hypermethylated with up-regulated expression,and CHGB,GRHL2,ALDH3B2,CDH1,DLX1,MUC6,MUCL1 and TFAP2B that were hypomethylated with down-regulated expression,indicating their potential involvement in the development of microtia.Conclusion This analysis of the differential pattern of m6A methylation in cartilage in microtia provides a theoretical basis for further study of the pathogenesis and potential treatment strategies of microtia.
Keywords：congenital microtiam6APPI networkEnrichment analysis
Publication Date：2025-06-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 438-447 )