Improvement of Gap-Prepulse Inhibition of Acoustic Startle in Evaluation of Tinnitus in Mice
TENG Qi
LIANG Wenqi
LI Yang
YU Qianru
WANG Tianying
LIU Shan
LI Chonghui
ZHOU Xuan
GONG Shusheng
LIU Ke
Abstract：Objective To construct a more stable and reliable mouse tinnitus model by improving the gap-prepulse inhibition of acoustic startle(GPIAS)models.Methods Six-weeks-old male C57BL/6J mice were randomly divided into a control and a noise exposure group.GPIAS was evaluated on days 1 and 7 in the control group and the difference calculated.The"tinnitus threshold"was determined based on the range of data fluctuation,which was applied to screen for tinnitus in the noise exposure group,and applied to control group data for the rate of false positive.Results The difference in GPIAS between day 1 and day 7 in the control group showed a normal distribution,with the tinnitus threshold calculated at 2δ=0.16.ABR thresholds at 4,8,12 and 16 kHz were elevated on day 1 after noise exposure(P<0.05),but not on day 7(P>0.05).On day 7 after noise exposure,7 animals showed GPIAS suggesting tinnitus,with reduced GPIAS%at 16 kHz compared to before noise exposure(P<0.01),but not at 4,8 and 12 kHz(P>0.05)nor with PPI%at 4,8,12 and 16kHz(P>0.05).GPIAS%and PPI%at 4,8,12 and 16 kHz showed no significant change in non-tinnitus mice after noise compared with before noise(P>0.05).On both day 1 and 7,one mouse in the control group demonstrated decreased GPIAS%more than the tinnitus threshold at 8 kHz,representing a rate of false positive at 6.67%.Conclusion The improved GPIAS evaluation can consistently and reliably identify tinnitus in mouse model with high accuracy.
Keywords：tinnitus evaluationtinnitus mousegap-prepulse inhibition of acoustic startle
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 108-112 )