Research on the Mechanism of Neutrophil Extracellular Traps in Otitis Media with Effusion in Children
SHEN Shimin
YANG Zhiqiang
XU Wen
HOU Yun
WANG Jinpeng
CHEN Kangbing
LIU Xiaohan
HE Jingya
CHEN Xiaowan
Abstract：Objective To investigate the role of neutrophil extracellular traps(NETs)in otitis media with effusion(OME)by studying their changes in middle ear effusion(MEE)and influencing factors.Methods Thirty-one patients with OME who underwent tympanotomy and/or tube placement at the Department of Otolaryngology Head and Neck Surgery,the First hospital of Lanzhou University,between 2021.01 to 2021.12,along with 27 cases of adenoid hypertrophy and 11 cases of sinusitis,were grouped based on disease duration,i.e.<3 months(14 cases),3-6 months(8 cases)and>6 months(18 cases).The levels of neutrophil elastate(NE),myeloperoxidase(MPO),cell-free DNA,interleukin-8(IL-8),and mucin 5B(MUC5B)in the MEE in each group were qualitatively and quantitatively analyzed by immunofluorescence,DNA content determination,and Western blot.Results NE and MPO were colocalized with cell-free DNA to form a network structure in all groups.Cell-free DNA,NE,MPO,IL-8 and MUC5B increased along with the duration of disease(P<0.05).Expression of MPO was positively correlated with NE(P<0.05),as well as for expression of MPO and NE and the expression of IL-8(P<0.05).Conclusion NETs are involved in the pathological process of OME and increase with the course of the disease,likely regulated by IL-8.The level of MUC5B in MEE in patients with OME shows an overall upward trend with the increase of the disease course.
Keywords：otitis media with effusionneutrophil extracellular trapsinterleukin-8mucin5B
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1011-1015 )