Cochlear Expression of FBXO3 in Noise Induced Hearing Loss in Rats and Its Significance
FANG Lian
LIN Bi
ZHAO Xiaohui
Abstract：Objective To investigate the expression of FBXO3 and autophagy related genes in the cochlea in rats with noise induced hearing loss and its significance.Methods Twenty Wistar rats with normal ear reflex were selected and randomly divided into a noise exposure group(narrow frequency band[100 Hz]centered around 12 kHz at 126 dB SPL for 2 hours)and a control group(no noise exposure),with 10 rats in each group.Auditory brainstem response(ABR)thresholds(dB SPL)were tested.Western blot was used to detect the protein expression of FBXO3 and LC3 in cochlear tissues.The expression of autophagy related genes ATG5 and LC3 in HEI-OC1 cochlear hair cell line was detected by knocking out the FBXO3 gene.Results In the noise exposure group,ABR thresholds increased at all frequencies,with decreased expression of FBXO3 and increased expression of LC3Ⅱ(P<0.01).With the FBXO3 gene knocked out in the hair cell line,the proportion of LC3Ⅱ/LC3I increased(P<0.05).After ubiquitination and degradation of MG132 inhibitory protein,the expression level of ATG5 in the cell line increased(P<0.01).Conclusion Following noise exposure,the expression of FBXO3 in the cochlea is inhibited in rats.Regulating the autophagy pathway and increasing the autophagy level may help protect cochlear cells and reduce noise induced hearing loss.
Keywords：autophagynoisehearing lossubiquitination
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1001-1005 )