Effect of Deamino Arginine Vasopressin on the Expression and Function of Mitogen-activated Protein Kinase Phosphatase-1 in Rat Cochlea
HU Tao
QU Yongtao
JIA Yuqi
XU Xia
GUO Mingli
Abstract：Objective The expression of mitogen-activated protein kinase phosphatase(MKP-1)in the cochlea of rats after the action of deamino arginine vasopressin(DDAVP)was examined to investigate whether MKP-1 is involved in the development of Meniere's disease.Methods Twenty male SD rats of SPF grade were selected and randomly divided into a study group and a control group(10 in each group).Animals in the study group received intraperitoneal DDAVP,while those in the control group an equal amount of intraperitoneal 0.9％NaCl.At the end of modeling,auditory brainstem responses(ABR)were obtained,morphological changes of the cochlea observed with hematoxylin-eosin staining,and the location of MKP-1 expression in the cochlea determined using quantitative immunohistochemistry and protein immunoblotting.Results ABR thresholds in the study group elevated by about 10 dB SPL after modeling(P<0.01),with endolymphatic hydrops on HE staining(80％)and widely expressed MKP-1 in the vascular striatum,spiral ligaments(SLM),spiral rims,Organ of Corti(OC)and the spiral ganglion on immunohistochemistry,showing significant difference between the two groups in the vascular stripe,spiral ligament and spiral ganglion(P<0.05)but not in SLM and OC(P>0.05).MKP-1 expression on protein immunoblotting was lower in the study group than in the control group(P<0.01).Conclusion DDAVP may be involved in the pathogenesis of Meniere disease by promoting endolymphatic hydrops formation through downregulation of MKP-1.
Keywords：deamino arginine vasopressinmitogen-activated protein kinase phosphatase-1meniere diseaseendolymphatic hydropsrat
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 996-1000 )