Effects of Simulated Microgravity on P120-catenin Expression in Rat Cochlear Basilar Membrane
ZHU Yinglei
WANG Gang
ZHOU Ying
WANG Lei
FAN Boya
HAN Haolun
LI Baowei
SUN Zhezhe
WU Jihua
WU Wei
Abstract：Objective To report effects of simulated microgravity on the expression of cochlear catenin cofactor p120-catenin(p120ctn)in the basilar membrane in rats.Methods Healthy male SD rats(n=48)were randomly divided into a simulated microgravity group and a control group(n=24 in each group),which were further divided subgroups based on exposure time(1 day,1 week,8 weeks and 12 weeks,n=6/12 ears in each subgroup.Prior to and immediately after exposure(B0 and P0),distortion product otoacoustic emissions(DPOAEs)and binaural auditory brainstem re-sponse(ABR)thresholds were obtained.At the conclusion of P0 audiometry,rats were executed and both cochleae iso-lated and stained immunohistochemically.Results No differences in DPOAE detection across the groups(P>0.05).ABR thresholds rose as exposure time increased and higher for 1 week,8 weeks and 12 weeks exposure than at B0(P<0.05).Increased P120-catenin expression was noticed in animals with 8 weeks or 12 weeks microgravity exposure as compared to controls(P<0.05).Conclusion Simulated microgravity negatively affects hearing in rats,with p120ctn ex-pression on cochlear basilar membrane increasing along with exposure time.Auditory function impairment brought on by microgravity may be influenced by p120ctn.
Keywords：microgravityauditory brainstem responseadhesion junction proteinp120-catenin
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 136-140 )