Arcn1b Deficiency Affects Auditory Function of Zebrafish by Inducing Endoplasmic Reticulum Stress
ZHENG Quan
WANG Guoqing
ZHAO Duyang
LU Feicai
SHI Shuai
Abstract：Objective To study the influence of arcn1b on auditory function in zebrafish and to provide basis for completing the clinical phenotypic spectrum of ARCN1-related syndromes.Methods Expression of arcn1b in the inner ear and neuromasts in zebrafish was confirmed by in situ hybridization.The zebrafish model of down-regulated arcn1b expression was established by the morpholino(Mo)technique.A Zebrafish behavior trajectory tracking system was used to detect the effect of down-regulated arcnlb expression on auditory behaviors.Changes of hair cells and supporting cells in the inner ear and neuromasts in zebrafish with down-regulation of arcn1b expression were recorded by immuno-fluorescence,and proliferation of supporting cells labeled with BrdU was analyzed in comparison with the control group.In situ hybridization was also used to evaluate the effect of down-regulated expression of arcn1b on the expres-sion of endoplasmic reticulum stress-related genes.Results Arcn1b was expressed in the inner ear and neuromasts.Down-regulation of arcn1b expression led to decreased stress ability to sound stimulation(P＜0.01),with decreased number of hair cells in the inner ear and neuromasts(P＜0.01),decreased number of body hair cell clusters(P＜0.01),de-creased number of neuromasts supporting cells in the head and decreased number of proliferative support cells in the head neuromasts(P＜0.05).Down-regulation of arcn1b expression also resulted in high expression of endoplasmic retic-ulum stress-related gene hspa5 in the head and neuromasts.Conclusions Lack of arcn1b may induce endoplasmic retic-ulum stress,thus inhibiting the proliferation of supporting cells,reducing the number of supporting cells and hair cells,and affecting the auditory function in zebrafish.Attention should be paid to hearing status in patients with ARCN1-relat-ed syndromes.
Keywords：zebrafishArcn1bhair cellsupporting cellendoplasmic reticulum stress
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 854-860 )