Fst attenuates Cochlear Inflammation and Oxidative Stress Via regulating the IκBα/NF-κB Signaling Pathway
LI Hongjin
ZHANG Yuanxiang
CHEN Peipei
ZHOU Ancheng
LIU Shaofeng
Abstract：Objective To investigate influence of follistatin(Fst)on inflammation and oxidative stress in cochlear basement membrane injury and the mechanisms.Methods In a model of neomycin induced cochlear basement mem-brane tissue injury,Fst expression was detected by RT-PCR.Changes of hair cells were observed under a fluorescence microscope with immunofluorescence staining.Inflammatory factors TNF-α,IL-1β and IL-6 were detected by ELISA.Oxidative stress index,IκBα,NF-κB P65 and expression of downstream targets were detected by Western Blot.Results Basement membrane hair cells were significantly damaged with neomycin injury.With Fst overexpression,hair cells conditions were significantly improved in the basal turn and partially preserved in the middle turn(P<0.05).Expression of TNF-α,IL-1β,IL-6,iNOS and COX-2 was increased with neomycin injury,while that of Fst was significantly de-creased.with decreased IκBα and NF-κB P65 in the cytoplasm,which were reversed following overexpression of Fst.P-IκBα also increased with neomycin injury,while was reversed after overexpression of Fst.In the nucleus,NF-κB P65 was significantly increased with neomycin injury and partially reversed by Fst overexpression.Conclusion Fst may re-duce cochlear inflammation and oxidative stress and protect cochlear hair cells by regulating the IκBα/NF-κB signaling pathway.
Keywords：FollistatinCochleaInflammationOxidative stressIκBα/NF-κB
Publication Date：2023-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 666-670 )