An Experimental Study of Correlations Between Gentamicin Induced Renal Trauma and Hearing Impairment

SUN KeBing; LIU Ke

doi:10.3969/j.issn.1672-2922.2017.03.012

Abstract：Objective To explore time and quantity properties of Texas Red labeling gentamicin entry into renal tissue and cochlear hair cells in mice, as well as secondary renal injury and hearing loss. Methods 18 adult C57 mice (6 wks old) were randomly divided into three groups. Two groups received daily intraperitoneal injection of gentamicin la-beled with Texas Red at 100 mg/kg for 1 and 7days, respectively. The third group only received the same volume of 0.9%saline for control. Laser confocal observations combined with Image-Pro Plus software were employed to estimate time and quantity of uptake gentamicin in both renal tissue and cochlear hair cells. Further, BUN, Scr, and auditory brainstem responses (ABR) threshold were examined to estimate the functional state of kidney and cochlea after genta-micin exposure. Results Significant increase of OD value at d1 was found in renal tissue compared with that of control (P<0.01);Significant , but lower increase of OD value at d1 was identified in cochlear hair cells compared with control (P<0.05);Similar remarkable enhancement of OD were found at d7 in both renal tissue and cochlear hair cells. More-over, significant increase of BUN and Scr were detected at d1 (P<0.05), however, no significant ABR (click) threshold elevation was found at d1 after treatment (P<0.05). Dramatic increases of BUN, Scr, and ABR threshold have been found at d7 compared with controls (P<0.01). Conclusions Aminoglycoside induced hearing impairment showed delay reaction compared with aminoglycoside induced rapid injury in renal tissue. The time delay may provide a possibility of clinical invention for aminoglycoside induced hearing loss.