Effects of high level white noise exposure on hearing function in C57BL/6J Mice
Wang Ding
Liu Ke
Zhang Yue
Yang Shiming
Ma Xiulan
Abstract：Objective To investigate changes of hearing threshold, morphology and the ribbon synapses in the co-chlear basilar membrane after 2 hours exposure to white noise at 120 dB SPL in C57BL/6J mice. Methods Twenty C57BL/6J mice with normal auditory thresholds were randomly divided into four groups (n=5, 10 ears, in each group). Animals in three groups (experiment groups) were examined at 0 day, 7 days and 14 days after noise exposure. One group was used as control. Immunofluorescent staining and scanning electron microscopy (SEM) examination focusing on morphology of OHCs and ribbon synapses were conducted after auditory brainstem response (ABR) testing. Result Auditory thresholds could not be detected at 0 day after noise exposure. Compared with the control, ABR thresholds re-mained elevated at 7 day after noise exposure (P<0.01). ABR thresholds at 14 days after noise exposure were better than at 7 days after, although still worse than the control (P<0.01). There was an obvious decrease in the number of ribbon synapsis. Adhesion and disarray of OHC stereocilia were seen on SEM. Conclusions Exposure to white noise at 120 dB SPL for 2 hours can lead to permanent threshold shift (PTS) in mice, causing severe damage to OHC and decrease of rib-bon synapses.
Keywords：PTSribbon synapsesNIHLC57BL/6J micehearing loss
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 217-221 )