Transduction efficiency and cytotoxicity evaluation of CMC-TAT nanocarriers in vitro
TANG Chaoying
HAN Weiju
DENG Xiongwei
WANG Fangyuan
YUAN Shuolong
YANG Shiming
WU Nan
Abstract：Objective To construct O-carboxymethy chitosan-TAT nanoparticles(CTNs), and to explore the efficiency and security of CTNs for miR96 by transfect 293 cells and cochlear basilar membrane. Method The CTNs were generated with the optimal weight ratio of 1.5:1 (CMC:TAT), which were used for the incorporation with miR-96 mimisc marked cy3. Transfect 293 cells and cochlear basilar membrane with Lipofectamine-miR96-cy3 and CTNs-miR96-cy3 at the concentra-tion of 150nM with control. The red fluorescence positive cells were counted to evaluate the efficiency of CTNs. The securi-ty of CTNs was evaluated by MTT assay. Result CTNs with a size of 186.8 nm and a zeta potential of-30.9 mV were suc-cessfully prepared. The transduction efficiency of CTNs-miR96-cy3 was lower than that of Lipofectamine-miR96-cy3 in both 293 cells and cochlear basilar membrane. MTT assay showed that the cytotoxicity of CTNs was significantly lower than that of Lipofectamine. Conclusion CTNs can successfully transduct miR96 in vitro, which reveals the feasibility and se-curity of CTNs as delivery vector for miRNA in cochlea,however, it’s transduction efficiency is low.
Keywords：O-carboxymethy chitosan-TAT NanocarriersmiRNAmiR96Transfection in cochlea
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 793-797 )