The responses of hereditary hearing loss genes to acoustic injury
YANG Shuzhi
CAI Qunfeng
DONG Youyi
YANG Weiping
HU Bohua
Abstract：Objective To determine the response of hereditary hearing loss genes to acoustic injury in mice. Methods Eight C57BL/6J mice (4 males and 4 females, 4 weeks old) were randomly divided into a control and a noise group. The noise group subjects were exposed to a broadband noise at 120 dB SPL for 1 hour. ABR thresholds were measured and miss-ing outer hair cells were quantified to determine the level of cochlear damage 1 day after noise exposure. qRT-PCR arrays were used to determine the expression levels of 92 genes that have been identified in human as hereditary hearing loss genes. Results ABR measurements showed a significant increase in threshold after the noise exposure (P<0.001) and pathological assessment revealed outer hair cell lesions in the basal turn of the cochlea. Gene expression analysis identified 6 genes that displayed significant expression changes. Among these genes, four (Col4a5, P2rx2, Tmc1, and Tprn) were down-regulated, and two (Ndp and Smpx) were up-regulated. These genes participate in various cellular functions. Conclusion Noise-induced cochlear damage is a complex degenerative process and multiple deafness genes are involved in its etiology.
Keywords：Acoustic injuryHereditary hearing lossGeneMousemRNA expression
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 719-724 )