Melting curve quantitative technology in quantification of mitochondrial DNA 1555A>G heteroplasmic mutation
GAO Hui
SHEN Shanshan
LIU Jingjing
WANG Linkai
LIANG Shaoming
ZHANG Ruanzhang
HU Yuhua
GUO Hui
LIN Yumei
WANG Shayan
Abstract：Objective The melting curve quantitative technology was established using the TaqMan probe and com-pared with DHPLC and Sanger sequencing technologies in detecting mtDNA 1555A>G heteroplasmic mutation. Methods TaqMan probe melting curve technology was used to test aminoglycoside-induced hearing loss families and normal individu-als. The results were compared with our previous results using DHPLC and Sanger sequencing technologies. Results The level of mutant mtDNA 1555A>G was detected by TaqMan probe melting curve technology successfully in 50 samples, identifying one more case than Sanger sequencing, but three less than DHPLC. Conclusions The quantity of mutant mtD-NA 1555A>G copies ranging from 19% to 86% was accurately detected by the TaqMan probe melting curve technology used in this experiment.
Keywords：mtDNA 1555A>Ghearing lossTaqMan probeMelting Curve AnalysisDHPLC
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 686-691 )