The Phenomenon of Autophagy in Gentamicin Induced Ototoxicity
LIU Dingding
LIU Yongze
MA Xiaofeng
LU Ling
ZHOU Han
QIAN Xiaoyun
GAO Xia
Abstract：Objective Gentamicin is a frequently used aminoglycoside antibiotic. With increasing dose and duration, its ototoxicity side effects will gradually appear. The current study aims to study the phenomenon of autophagy in gentami-cin induced ototoxicity. Methods The cochlea was cultured with gentamicin exposure. Transmission electron microscopy, immunofluorescence and Western blotting were used to observe the expression of autophagy protein LC3 in the explant cul-ture treated with the gentamicin. Results In vitro administration of gentamicin in mouse cochlear cultures induced the for-mation of autophagic vesicles and autophagosomes in hair cells. Conclusion Our results demonstrate that autophagy partici-pates in the process of ototoxicity induced by gentamicin and suggest that intervention aimed at autophagy may influence the ototoxicity process.
Keywords：Hair cellsGentamicinOtotoxicityAutophagy
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 260-264 )