Expression of HSP60 in a rat model of drug-induced deafness
TIAN Keyong
SONG Yongli
SUN Fei
WANG Renfeng
YUE Bo
WANG Min
CHEN Fuquan
QIU Jianhua
Abstract：Objective To study the expression of heat shock protein 60 (HSP60) in rat cochleae and its changes in ka-namycin monosulfate (KM)-induced ototoxicity. Methods Twenty adult female Sprague-Dawlay (SD) rats were randomly divided into two groups (n=10 in each) to receive intraperitoneal injection of KM (500 mg/kg qd) for twenty one days, or equivalent volume of saline (control group). ABR tests were used to detect hearing loss. The pattern and level of HSP60 ex-pression within the cochlea were tested by real-time PCR, Western blot and immunofluorescence assays. Results ABR tests showed significantly higher thresholds in the experimental group than in the control group (P<0.05). Real-time PCR,West-ern blot and basilar membrane immunofluorescence staining showed decreased expression of HSP60 in the experimental group (P<0.05). Immunofluorescence staining of frozen cochlear sections revealed HSP60 expression in supporting cells of the organ of Corti. Conclusion HSP60 is expressed in supporting cells under normal conditions, but is decreased in rats with chronic drug-induced hearing loss. It is hence speculated that HSP60 may participate in the process of drug-induced hearing loss.
Keywords：HSP60Hearing lossSupporting cell
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 251-254 )