Association of ICAM-1 gene polymorphism with sudden sensorineural hearing loss

ZHANG Fang; TIAN Guangyong; DUAN Yongchang; YANG Jingya; TANG Ling

doi:10.3969/j.issn.1672-2922.2015.04.037

Abstract：Objective To determine the relationship between the 469K/E polymorphism (rs5498) of the ICAM-1 gene and sudden sensorineural hearing loss. Methods Subjects used in this study were derived from the Third Affiliated Hospital of Southern Medical University. Two milliliters of venous blood were obtained from each participant for genomic DNA extraction. Detection of SNPs rs5498 was performed by polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) analysis. The restriction enzyme-digested PCR products were analyzed by 2% agarose gel electrophoresis. The Chi-square test was used to compare the frequency of alleles and genotypes between case and control groups. Results Seven-ty-five SSNHL patients (39 male and 36 female patients) were compared with 165 controls (86 male and 79 female subjects) enrolled in this study from June 2014 to July 2015. No significant differences in ICAM-1 469K/E genotype and allele frequencies were found between the SSNHL group and controls. However, there were significant differences in ICAM-1 469K/E polymorphism between patients with different types of hear-ing loss. Conclusion Our study results do not show significant association between 469K/E polymorphism of the ICAM-1 gene and sudden sensorineural hearing loss. The E allele may influence the susceptibility to the development of flat type of hearing loss.