Different patterns of endocytosis identified in murine cochlear inner and outer hair cells
LI Sijun
LIU Ke
TANG Siquan
Yang Shiming
Abstract：Objective To report different patterns of endocytosis in murine cochlear inner and outer hair cells (IHCs&OHCs). Methods Normal C57BL/6J mice (1 month old) were used to observe different patterns of endocytosis in IHCs and OHCs in vitro using live cell imaging of FM1-43. Results Intense fluorescence den-sity of FM1-43 indicating massive endocytosis activities was identified at the base and blow the nucleus in IHCs. In contrast, major endocytosis activities in OHCs were found at or near the lateral cellular wall and above the nucleus. FM1-43 uptake in IHCs was higher than that in OHCs (P<0.05). Conclusion Active endocy-tosis at or near the basolateral area of the IHC suggests that endocytosis in IHCs may involve ribbon synapse plasticity. Endocytosis activities near the lateral wall and in the supranuclear area in OHC indicate that endocy-tosis in OHC involves tip-links or iron channels. Higher levels of FM1-43 uptake by IHCs may indicate IHC’s key roles in hearing development and maintenance.
Keywords：EndocytosisInner and outer hair cellFM1-43Ribbon Synapse
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 545-548 )